肖志梁,杨芳芳,韦章良,邱晨,黄怡,董俊德,龙丽娟.海水酸化和升温对孔石藻(Porolithon onkodes)共附生细菌及其诱导珊瑚幼虫附着的影响[J].海洋通报,2022,(6): |
海水酸化和升温对孔石藻(Porolithon onkodes)共附生细菌及其诱导珊瑚幼虫附着的影响 |
The impacts of elevated pCO2 and temperature on Porolithon onkodes associated bacterial community and on their function that facilitating coral settlement |
投稿时间:2021-11-30 修订日期:2022-12-02 |
DOI:10.11840/j.issn.1001-6392.2022.06.008 |
中文关键词: 酸化 升温 孔石藻 鹿角杯形珊瑚 幼虫附着 细菌群落 |
英文关键词:acidification warming Porolithon onkodes Pocillopora damicornis larval settlement bacterial community |
基金项目:国家自然科学基金(42176157);中国科学院重点部署项目(ZDRW-XH-2021-02-06);南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0402);中国科学院战略性先导科技专项(A类)(XDA13020203) |
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中文摘要: |
珊瑚藻是珊瑚礁生态系统中一类主要的钙化生物,不仅能够构建、稳固礁体,而且与造礁石珊瑚关系密切,其对造礁石珊瑚幼虫附着的促进作用是退化石珊瑚群落恢复的关键。但是,面对未来气候变化,珊瑚藻对珊瑚幼虫的诱导作用会如何响应,以及该过程是否与藻体共附生细菌群落有关,目前尚不清楚。本文选取了广泛分布于我国南海珊瑚礁生态系统中的孔石藻(Porolithon onkodes)为研究对象,分别采用不同pCO2和温度培养孔石藻31 d,研究不同处理后孔石藻对鹿角杯形珊瑚幼虫附着的影响,并通过分析藻体共附生细菌群落的变化情况,探究细菌多样性与孔石藻对珊瑚幼虫诱导功能的关系。结果表明,与对照组相比,酸化(1200 ppm/1800 ppm pCO2)和升温(30 ℃/32 ℃)均没有显著影响孔石藻诱导鹿角杯形珊瑚(Pocillopora damicornis)幼虫附着,其中温度30 ℃,pCO2 1200 ppm时,珊瑚幼虫附着率最高100.00%。采用16s rRNA高通量测序技术分析了不同处理组孔石藻共附生细菌群落结构及多样性。结果表明,孔石藻共附生细菌主要由变形菌门、拟杆菌门和绿弯菌门等菌群构成,其中变形菌门含量达到40.34%~73.45%;与酸化相比,孔石藻共附生细菌群落对温度变化更敏感,其中32 ℃处理组具有更高的细菌多样性,但经Kruskal-wallis秩和检验显示不同温度和CO2浓度处理组,在门、纲、属水平上细菌多样性、丰度及组成均无显著性差异,说明孔石藻共附生细菌群落结构相对稳定,推测这可能与孔石藻诱导珊瑚幼虫附着不受温度和酸化影响密切相关。研究结果对预测未来气候变化对珊瑚礁生态系统的影响、制定珊瑚礁生态系统保护与修复策略具有重要的理论价值和生态意义。 |
英文摘要: |
Crustose coralline algae (CCA) play vital ecological roles in coral reef ecosystem, due to their participation in the formation and stabilization of coral reefs and roles as permanent settlement substratum for many marine invertebrate larvae. Several studies have indicated that CCA are sensitive to ocean acidification and global warming, but little is known about the impacts of interaction between reduced pH and elevated temperature on inductive capacity and associated bacterial assemblages of CCA. Here, we investigated the effects of Porolithon onkodes on larval settlement, metamorphosis and survival rate of Pocillopora damicornis under ocean acidification and warming within 31 d. The results revealed that acidification (1200 ppm/1800 ppm) and warming (30 ℃/32 ℃) had no significant impacts on facilitation of CCA to coral settlement. When P. onkodes was exposed to 30 ℃ and pCO2 1200 ppm, larval settlement rate was 100.00%. Furthermore, algal associated bacterial community was analyzed by amplicon pyrosequencing of the V3–V4 region of 16s rRNA. The results suggested that bacterial community was more sensitive to elevated temperature compared with acidification; however, there were no significant differences in composition and diversity of bacterial community at phylum and genus level. It suggested that the bacterial community associated with P. onkodes was relatively stable, which may be related with the invariability of algal inductive capacity. The study may contribute to predicting the effects of climate changes on reefs, and protecting and restoring reefs. |
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