庄丽雯,周文静,毛奕淋,郭赣林,王长海,孙颖颖.大型海藻中环二肽类抑藻活性化合物的分离纯化[J].海洋通报,2022,(6):
大型海藻中环二肽类抑藻活性化合物的分离纯化
Isolation and purification of diketopiperazines with antialgal activity from marine macroalgae
投稿时间:2022-01-15  修订日期:2022-02-24
DOI:10.11840/j.issn.1001-6392.2022.06.009
中文关键词:  龙须菜  条斑紫菜  抑藻活性  环二肽  分离纯化
英文关键词:acidGracilaria lemaneiformis  Porphyra yezoensis  antialgal activity  diketopiperazines  isolationification  warming  Porolithon onkodes  Pocillopora damicornis  larval settlement  bacterial community
基金项目:国家自然科学基金-青年基金项目(41606120);江苏省自然科学研究-面上项目(BK20211353);2018年江苏高校优秀中青年教师和校长境外研修计划;连云港市“海燕计划”资助项目(2020-QD-020);江苏省优势学科建设工程资助项目。
作者单位E-mail
庄丽雯 江苏省海洋生物技术重点实验室 江苏海洋大学 江苏 连云港 222005 1786653398@qq.com 
周文静 江苏省海洋生物技术重点实验室 江苏海洋大学 江苏 连云港 222005  
毛奕淋 江苏省海洋生物技术重点实验室 江苏海洋大学 江苏 连云港 222005  
郭赣林 江苏省海洋生物技术重点实验室 江苏海洋大学 江苏 连云港 222005江苏省海洋生物产业技术协同创新中心 江苏海洋大学江苏 连云港 222005江苏省海洋生物资源与生态环境重点实验室 江苏海洋大学 江苏 连云港 222005  
王长海 资源与环境科学学院 南京农业大学 江苏 南京 210095  
孙颖颖 江苏省海洋生物技术重点实验室 江苏海洋大学 江苏 连云港 222005江苏省海洋生物产业技术协同创新中心 江苏海洋大学江苏 连云港 222005江苏省海洋生物资源与生态环境重点实验室 江苏海洋大学 江苏 连云港 222005 syy-999@163.com 
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中文摘要:
      采用茚三酮试剂显色的薄层原位化学反应和抑藻活性检测方法,从龙须菜、条斑紫菜、鹿角藻和裙带菜中筛选环肽,发现龙须菜和条斑紫菜中存在具有抑藻活性的环肽。以龙须菜和条斑紫菜粉末为原料,采用硅胶柱层析和(或)硅胶薄层层析制备等方法,从龙须菜中分离到环(脯氨酸-甘氨酸)、环(丝氨酸-脯氨酸)和环(丙氨酸-色氨酸),从条斑紫菜中纯化到环(亮氨酸-脯氨酸)和环(缬氨酸-酪氨酸)。除环(丝氨酸-脯氨酸)外,其余4种环二肽均是首次从相应大型海藻中获得。环(脯氨酸-甘氨酸)、环(亮氨酸-脯氨酸)和环(缬氨酸-酪氨酸)明显抑制了强壮前沟藻、赤潮异弯藻和球形棕囊藻的生长,对米氏凯伦藻和中肋骨条藻有选择性抑制作用。在50 μg/mL时,3种环二肽对强壮前沟藻、赤潮异弯藻和球形棕囊藻的生长抑制率≥50%(第4d);环(脯氨酸-甘氨酸)对米氏凯伦藻以及环(亮氨酸-脯氨酸)对中肋骨条藻的生长抑制率超过或接近50%。随后,获得环二肽对赤潮微藻生长的半抑制效应浓度EC50-96h。结果表明,环(脯氨酸-甘氨酸)、环(亮氨酸-脯氨酸)和环(缬氨酸-酪氨酸)在抑制米氏凯伦藻、球形棕囊藻或赤潮异弯藻方面比重铬酸钾更有优势,有望开发为环境友好型赤潮微藻抑藻剂。
英文摘要:
      The cyclic peptides with antialgal activity were screened from Gracilaria lemaneiformis, Neoporphyra yezoensis, Silvetia siliguosa and Undaria pinnatifida, by thin-layer in-situ chemical reaction with ninhydrin reagent and antialgal activity test. The cyclic peptides with antialgal activity were found in the extracts of Gracilaria lemaneiformis and Neoporphyra yezoensis, respectively. Cyclo (Pro-Gly), cyclo (Ser-Pro), cyclo (Ala-Trp) were finally isolated from Gracilaria lemaneiformis, and cyclo (Leu-Pro) and cyclo (Val-Tyr) were purified from Neoporphyra yezoensis by repeated silica gel column chromatography and (or) silica gel thin layer chromatography. Except for cyclo (Ser-Pro), the other four cyclic dipeptides were obtained from corresponding marine macroalgae for the first time. Cyclo (Pro-Gly), cyclo (Leu-Pro) and cyclo (Val-Tyr) significantly inhibited the growth of Amphidinium carterae, Heterosigma akashiwo, and Phaeocystis globosa; they showed selective growth inhibition for Karenia mikimotoi and Skeletonema costatum. At the concentration of 50 μg/mL, the growth inhibition of cyclo (Pro-Gly), cyclo (Leu-Pro) and cyclo (Val-Tyr) for Amphidinium carterae, Heterosigma akashiwo and Phaeocystis globosa was ≥50% (on?the?4th?day); the growth inhibition of cyclo (Pro-Gly) for Karenia mikimotoi and that of cyclo (Leu-Pro) for Skeletonema costatum were more than or close to 50%. Further, EC50-96h of cyclic dipeptide for red tide microalgae was obtained. EC50-96h of cyclo (Pro-Gly) for Karenia mikimotoi and Phaeocystis globosa, EC50-96h of cyclo (Leu-Pro) and cyclo (Val-Tyr) for Heterosigma akashiwo were less than that of potassium dichromate for corresponding red tide microalgae. This suggested that cyclo (Pro-Gly), cyclo (Leu-Pro) and cyclo (Val-Tyr) have more advantages in inhibiting Karenia mikimotoi, Phaeocystis globosa or Heterosigma akashiwo. And they are expected to be developed into an environment-friendly algal inhibitor against red tide microalgae.
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